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    TAN Ying-xia, YUE Liang, ZHANG Ke
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    Organoid technology is a new frontier technology in the field of life sciences. An organoid is now defined as a 3D structure derived from either pluripotent stem cells (PSCs) or organ-restricted adult stem cells (ASCs) and critically dependent on the multi-lineage differentiation and self-organization potential of stem cells in culture. Most excitingly,many recent studies describe organoids as consisting of organ-specific cell types,exhibiting key structural and functional properties through cell sorting and spatially restricted lineage commitment. Human organoids can overcome the disadvantages of traditional biomedical models-2D cell lines and animal models,thus filling the gap of ex vivo human tissue accessibility and related ethical considerations. The rapid advancement of organoid technology revolutionizes biomedical research,promises exciting new insights into developmental biology and opens new routes for necessary clinical approaches to treating human diseases and regenerative medicine. In this review,we discuss the definition of organoids,origin of organoid technology,potential clinical translation applications and existing challenges.
  • Original articles
  • Original articles
    ZHANG Ling, ZHENG Chuan-fen, RONG Hong-hui, LU Lu, PENG Yi, CHEN Ji-an
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    Objective To find out about the current literacy level and awareness rate of health among recruits in a unit,and analyze the influencing factors so as to provide reference for formulating programs for health education and health promotion. Methods A total of 1846 recruits were surveyed via the China Citizen Health Literacy Questionnaire compiled by China Health Education Center in 2015,involving three dimensions--basic health knowledge and theory, healthy lifestyle and behavior, and fundamental skills and involving six types of problems--scientific health concepts,infectious disease prevention and treatment,chronic disease prevention and treatment,safety and first aid,basicmedical care, and health information. χ2 test and multivariate unconditional logistic regression analysis were used. Results The overall level of health literacy among these new recruits was 8.88%,the score of health literacy was 37.07±12.45,and the health literacy awareness rate was 60.56%. Among the three dimensions,the level of life-styles and behavior was the lowest,which was 10.18%. Among the six types of problems,the literacy level of chronic disease prevention and control was the lowest(9.75%). The awareness rate of health information was the lowest,which was 48.91%. There were 24 items(48% of the total) where the awareness rate was less than 60%. Among them,the awareness rate of the benefits of eating soybean was the lowest(9.53%),followed by descriptions of the liver(only 10.78%). Multiple Logistic regression analysis showed that the health literacy level of females was higher than that of males (OR=2.407,95%CI:1.430-4.054). Compared with urban areas, the health literacy level in rural areas was lower(OR=0.613,95%CI:0.436-0.862). The health literacy level of recruits with a college education and above was the highest (OR=1.799, 95%CI:1.216-2.664).And the health literacy level of mothers who were farmers was the lowest (OR=0.491, 95%CI:0.303-0.797). Conclusion The health literacy level of new recruits is low,especially regarding the prevention and treatment of diseases,suggesting that the military should make related knowledge more accessible to recruits and enforce health interventions.
  • Original articles
    LU Hua-shan, LIU Fu-xiang, XIONG Yi, ZHANG Yu-fen, NIU Jian-ben, BIAN Xiao-jie, YUE Li-qun
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    Objective To investigate the current health literacy of grass-roots troops at a military unit, analyze the influencing factors, and provide data for targeted health education. Methods The "National Residents' Health Literacy Monitoring Questionnaire" was issued to ordinary soldiers at a military unit to find out about their health literacy. Based on single-factor group comparison and multi-factor Logistic regression analysis, the influencing factors were identified. Results The health literacy of these soldiers was 22.81%. Literacy was the highest with basic knowledge and beliefs(37.59%), followed by healthy lifestyles and behavior (28.44%), and health skills (22.12%). Literacy of the six types of problems was the highest with health information (74.99%), followed by safety and first aid (73.98%), awareness of health(41.27%), basic health care(25.06%), chronic disease prevention(22.75%) and infectious disease prevention (22.16%). Univariate analysis showed that gender, age, levels of education, marital status, smoking status, chronic diseases, family members with chronic diseases and self-assessed health status were statistically different between different groups. The difference in the health information literacy of people with different self-assessed health status, and in the safety and first aid literacy of smokers and non-smokers was P=0.001. The difference in the literacy of groups chronic disease prevention among people with different chronic diseases was P=0.011. The difference between other was P<0.001. There were statistically significant differences in on education levels of parents, locations of families, and ethnic groups. Multi-factor Logistic regression analysis showed that gender, age, education levels, smoking status, chronic diseases, chronic diseases among family members, and self-assessed health status were independent influencing factors of health literacy. Conclusion The factors that affect health literacy include gender, age, education levels, smoking status, chronic diseases, and self-evaluated health status.
  • Original articles
    ZHAO Hai-bo, NING Jie, GUO Jian-feng
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    Objective To analyze the changes in heart rate (HR), systolic blood pressure (SBP), diastolic blood pressure (DBP), pulse oxygen saturation (SpO2) and acute high-altitude reaction among healthy male soldiers who entered the plateau from a medium altitude area for the first time in order to provide reference for health support during military missions. Methods HR, SBP, DBP and SpO2 were monitored for 30 days for the 69 healthy soldiers who had entered the 4400-meter plateau from a 1500-meter area. Questionnaires on acute mountain sickness (AMS) were also filled out and scored by the Lake Louise AMS scoring system (LLS) (edition 2018). Results The changes in HR, SBP, DBP and SpO2 reached the peak six days after they entered the plateau, and the vital signs were gradually stabilized after 30 days of acclimatization, but no vital signs except for SBP returned to the original levels. Thirty-seven of the 69 participants (53.62%) developed AMS, and the main symptoms were headache, fatigue or weakness and decline in activity, dizziness, difficulty sleeping and loss of appetite. However,after the initial acclimatization (30 days), sleep disorders (65.22%) and gastrointestinal symptoms (55.07%) became the most common symptoms. Conclusion The incidence of AMS on plateaus among people who used to live in medium-altitude areas is lower compared with those from the plains. Due to hypoxia stimulation, the vital signs will gradually become stabilized on the plateau after experiencing sudden changes, but it is difficult to reach the medium-altitude level in the short term. After one month of plateau acclimatization, officers and soldiers can generally adapt to the plateau environment well enough to conduct physical training of appropriate intensity.
  • Original articles
    XUE Xiao-fang, LI Wei-wei, WANG Xin-yun, GENG Guo-tong
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    Defense Advanced Research Projects Agency(DARPA) is the organization responsible for the basic, leading and subversive scientific and technological breakthroughs in US defense technology, and plays an important role in promoting a new round of military revolution. This article makes an in-depth analysis of DARPA's biological crossover technology programs from 2007 to 2022, and studies the transformation,application and developments so as to provide reference for China's research on national defense biotechnology.
  • Original articles
    ZHU Hua-jian, WANG Jing-yi, ZHOU Zheng-yi, XIAO Zhong-hai, DONG Qing-yang, DU Fei, LI Zhe
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    Objective To analyze literature related to heat stress in order to find out more about the evolution of research, key authors and institutions, core journals, hot spots and developments in this field. Methods English and Chinese literature related to heat stress documented in Web of Science databases and China Knowledge Network (CNKI) was retrieved. The number of publications, author-institution collaboration view, country collaboration view, co-occurrence of keywords and keyword timeline knowledge map of related literature were generated and analyzed via Bibliometrix and VOSviewer. Results A total of 5149 articles in english and 937 articles in Chinese were retrieved. This field had been gaining attention since the 1990s, and the United States was the country with the largest number of publications and citations in this field. The cooperation between domestic research institutions or authors was more fragmented and no obvious clustering communities had been formed. The analysis results of key words showed that the effect of heat stress on the physiological function of the body, mechanisms related to thermal adaptation and heat shock proteins were hot spots at home and abroad. In China, research on the oxidative stress response to heat stress continued to increase in recent years,but more attention was paid to the effects of global warming on heat stress and the countermeasures. Conclusion This knowledge mapping visualizes the research on heat stress, locates the leading research teams in this field, and analyzes the current research and developments.
  • Original articles
    HUANG Cui, LIANG Hui-gang
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    The global pandemic of COVID-19 has fully exposed and exacerbated the inadequacy of global biosafety capabilities to respond to emergency epidemics. Assessment and analysis of the status quo and problems with global biosafety capabilities to respond to emergency epidemics is of great significance for effectively improving global biosafety preparedness and responding to the next epidemic. This article assesses the deficiencies of global biosafety capabilities to respond to emergency epidemics based on the Global Epidemic Preparedness Index (EPI) and Global Health Security Index (GHSI) released before the COVID-19 pandemic, analyzes the problems with biosafety facing each country, and proposes strategies to improve biosafety capabilities to respond to emergency epidemics.
  • Original articles
    LI Jian, CAO Yan, CHEN Ying, WANG Yu-fei, WU Li-ying
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    Objective To investigate the roles of autophagy and autophagy-related proteins ATG5/ATG7 in the expansion of neural stem cells (NSCs) in vitro. Methods NSCs were isolated from the brains of embryonic SD rats and cultured in vitro. During the expansion of NSCs in vitro, the changes in the diameter of neurospheres were observed, the cell viability was detected by CCK-8 kit, the protein expression levels of ATG5, ATG7, LC3 and PCNA were analyzed by Western blotting assay,the formation of autophagosomes was observed under a transmission electron microscope (TEM), the expressions of LC3 and Ki67 were analyzed by immunofluorescence, and the cell proliferation ability was tested by EdU assay. The role of autophagy and ATG5/ATG7 in the expansion of NSCs was evaluated after Baf A1 treatment or knockdown of Atg5/Atg7 with siRNA. Results Autophagy and proliferation were gradually enhanced with the expansion of NSCs in vitro. The proliferation of NSCs was significantly inhibited after autophagy was blocked with Baf A1, indicating that the increase in autophagy could promote the proliferation of NSCs. In addition,compared with the control group, autophagy and proliferation of NSCs decreased significantly after knockdown of Atg5 or Atg7. Conclusion The expansion of NSCs in vitro can be promoted by autophagy,in which ATG5 and ATG7 are critical to autophagy of NSCs.
  • Original articles
    ZHOU Yao, RAN Xiao-ju, ZHANG Jian, JIA Xue, HAO Ning, SUN Wei
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    Objective To explore the effect of minoxidil on the proliferation, migration and invasion of hepatocellular carcinoma (HCC) cells and the possible molecular mechanism. Methods The effects of minoxidil on cell proliferation, migration,invasion and apoptosis were detected by CCK-8 assay,wound-healing assay,Transwell assay and flow cytometry after minoxidil treatment of human HCC cell lines Huh7 and MHCC97H. Quantitative proteome profiling was performed to analyze the protein expression differences in cancer cells after minoxidil treatment. And the properties of differentially expressed proteins were revealed by enrichment analysis of the biological process, cellular component and molecular function. Pathway alterations were also analyzed based on KEGG database annotations. Results Minoxidil inhibited the proliferation, migration and invasion of HCC cells, without significant effect on apoptosis. There were 279 differentially expressed proteins identified in MHCC97H cells after minoxidil treatment. Among them, 89 proteins were up-regulated and 190 were down-regulated. The differentially expressed proteins were mainly involved in such biological processes as cell-cell adhesion, oxidation-reduction processes, DNA replication, and signaling pathways including ribosome biogenesis in eukaryotes, steroid biosynthesis and DNA replication. The expressions of both intracellular and extracellular proteins were affected. Conclusion Minoxidil can inhibit the proliferation, migration and invasion of HCC cells in vitro. Proteomic analysis showed that minoxidil might play an anti-tumor role in HCC by affecting the important proteins and pathways related to tumor proliferation, migration and invasion.
  • Original articles
    LI Yi, LIU Xiao-feng, WANG Bo, CHAI Wei-li, WANG Xue-you, ZHANG Wei, CUI Shu-jie
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    Objective To design and develop a combined oxygen production system combined with oxygen-enriched membrane and pressure swing adsorption(PSA),and to explore the oxygen production process of the two oxygen production modes combined. Methods Based on theoretical calculations,3D design and performance testing,the structure and oxygen production process of the two oxygen production modes were optimized,and the performance of the oxygen production system was verified. Results The oxygen production system with oxygen-enriched membrane and PSA could achieve an oxygen production concentration of 95%. Conclusion Compared with the PSA oxygen production device,the combined oxygen production process of oxygen-enriched membrane followed by PSA can increase the oxygen production concentration by more than 2% as long as the oxygen output pressure and oxygen production remain stable.
  • Reviews
  • Reviews
    LI Feng, WANG Ying, WANG Xue, ZHAO Yun, XIE Fang, QIAN Ling-jia
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    Cognitive dysfunction is a chronic neurological disease caused by complex interactions between a wide range of factors, causing great harm to patients and the surrounding community. The pathogenesis of cognitive dysfunction is complicated and diverse, including demographic factors, genetic factors, lifestyle and personal history. With the increase in social and economic stresses, stress-induced cognitive impairment has gradually attracted more attention. Homocysteine(Hcy), as an independent risk factor for cognitive dysfunction, has become of great interest in the medical community. This review traces the research progress in chronic stress-induced Hcy elevation and summarizes the biological mechanisms of chronic stress and Hcy impairment of cognitive function in order to explore the possible role of Hcy in stress-induced cognitive dysfunction and to provide reference for the prevention of and intervention in stress-related cognitive dysfunction.
  • Reviews
    LÜ Ruo-mei, WANG Jing, XIN Wen-wen, KANG Lin, SONG Yu-zhu
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    Botulism is caused by the ingestion of botulism neurotoxin, with rapid disease onset and potentially high mortality. Botulism is mainly treated with equine derived botulinum antitoxin. Recently, many new genetic engineering antibody medicines are also used to cure botulism, such as nanobodies, which are becoming increasingly popular as reagents for biomedical research for therapeutic use in recent years, characterized by a small relative molecular mass, good stability, high antigen affinity and easy production and transformation. A number of studies have reported the outstanding neutralizing effect of anti-botulinum toxin nanobodies. However, the main disadvantage of nanobodies in clinical treatment is its short half-life period, so many studies have focused on prolonging the half-life of nanobodies, including polyethylene glycol (PEG) conjugation, coupling to red blood cell carriers, fusing with the Fc segment of immunoglobulin G and HSA protein. This article reviews the screening, preparation, and modification methods of nanobodies against botulinum neurotoxins, which can provide support for subsequent research on botulinum neurotoxin nanobodies.
  • Reviews
    YUE Wen-yi, SHENG Fu-geng
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    Breast cancer is one of the most common malignancies in women. Early diagnosis and treatment is the key to good prognosis. As a new technology, artificial intelligence(AI) is developing rapidly. Its application in the field of imaging and pathology has achieved remarkable results and promised great potential and applicability. MRI is the examination technology with the highest resolution of soft tissue and the most imaging information among breast cancer imaging diagnosis methods. AI assisted breast cancer MRI has shown good performance in many fields, providing novel ideas for accurate diagnosis and treatment of breast cancer. This article reviews the research progress in AI in breast cancer MRI.
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