RSSE-mail Alert
Military Medical Sciences

Current issue

  • Select all
    |
    Guideline and Consensus
  • Guideline and Consensus
    Expert Consensus on Principles of Diagnosis and Prevention of Military Training Injuries （2022 edition）
    CHANG Qi, LI Chun-bao, HE Jie, TANG Liang, REN Hong-feng, ZHU Lü-gang, ZHANG Liang, XUE Zhi-chao, ZHANG Wei-xu, LI De-zhao, YANG Di, WANG Long, LI Shao-guang, TANG Pei-fu
    2022, 46(9): 641-646. https://doi.org/10.7644/j.issn.1674-9960.2022.09.001
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Military training injuries are a common and frequently occurring disease in the army， which pose a serious risk to the health of soldiers and combat effectiveness. On the basis of the larger number of pilot projects for military training injury prevention and control and the "three tours" carried out by the whole army， the research group organized experts in related fields of military training injury prevention and control to compile the Expert Consensus on Principles of Diagnosis and Prevention of Military Training Injuries （hereinafter referred to as the "Consensus"） in line with the principles of putting prevention first， taking into account the reality， highlighting key points and proper rehabilitation by reference to the Regulations on Health Protection in Military Training. The "Consensus" has explicitly specified the definition， diagnostic basis， diagnostic criteria， classification criteria for injuries， diagnostic points， treatment principles and prevention principles of military training injuries. It aims to guide military surgeons at each level to effectively carry out early prevention， standardized diagnosis， treatment and rehabilitation intervention of military training injuries， which will play an important role in the prevention and treatment of military training injuries in the whole army.
    • Original articles
  • Original articles
    Human tissue equivalent spheres for organ dose measurement of solar particle events in space
    ZHANG Xiao-dong, LU Wei, ZHAO Ya-li, XING Xing-he, GUO Yao-yu, LI Na
    2022, 46(9): 647-652. https://doi.org/10.7644/j.issn.1674-9960.2022.09.002
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Objective To study a human tissue equivalent sphere suitable for proton organ dose measurement in order to accurately measure the dose of organs during acute radiation risk assessment of solar particle events （most of which are protons） in manned space missions. Methods Based on our previous research on radiant phantom, the overall dimension, density, element proportion and proton energy loss rate of the Astronaut Center of China （ACC） equivalent sphere were obtained by means of measurement and simulation and compared with the values recommended by the International Commission on Radiation Units and Measurements （ICRU）. Finally, the radiation doses of 10-200 MeV protons at different depths of the ICRU equivalent sphere and ACC equivalent sphere were calculated with Geant4 software to verify the equivalence of dose distribution. Results Compared with the ICRU equivalent sphere, the material density deviation of the ACC equivalent sphere was 0.6%, the content deviation of hydrogen element was 1.78%, and the proton energy loss rate deviation was less than 2%. The proton dose deviations at the depths of 0.07, 3, 10, 15,30,50,75 and 100 mm were 8.0%, 10.8%, 12.6%, 19.4%, 25.0%, 17.0%, 22.8% and 16.2% respectively, and the overall deviation was 16.5%. Conclusion The proton dose measurement performance of the ACC equivalent sphere can fulfill the requirements of radiation protection. This sphere can be used as an effective means to provide measurement data for risk assessment of solar particle events.
  • Original articles
    Comparison of effect of different cell activators on activation, proliferation, expression of chemokine receptors, and cytotoxicity of CD8+T cells
    DENG Ru, WANG Xiao-hui, ZHANG Yu-long, SUN Su-jing, ZHAN Lin-sheng
    2022, 46(9): 653-658. https://doi.org/10.7644/j.issn.1674-9960.2022.09.003
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Objective To compare the effect of ConA and Anti-CD3/28 on activation, proliferation, expression of chemokine receptors and cytotoxicity of CD8+T cells,in order to provide experimental basis for selecting an efficient and uniform activator of CD8+T cells. Methods C57BL/6 mice were euthanized and their spleens were separated. CD8+T cells were isolated with magnetic beads from spleens before being activated with ConA or Anti-CD3/28 respectively. The effect on CD8+T cells was estimated. FCM was adopted to determine the apoptosis, activation, and expression of chemokine receptors of these cells. Multi-analyte flow assay was used in the cytokines test. A subcutaneous tumor model was employed in cytotoxicity assay. Results CD8+T cells activated by ConA [（19.7± 1.0）× 106] had a higher proliferation ability than those in the Anti-CD3/28 group, and showed a higher expression level of CD69, which is a biomarker of T cell activation at the early stage. There was little difference between the two candidates in expressions of chemokine receptors of CD8+T cells. However, the Anti-CD3/28-activated CD8+T cells exhibited more robust cytotoxic effect on E.G7 cells in vitro and in vivo. Conclusion CD8+T cells activated by Anti-CD3/28 have significant cytotoxicity on E.G7 cells and tumor, which demonstrates that Anti-CD3/28 is more efficient in activating cytotoxic T cells in vitro and in vivo.
  • Original articles
    A preliminary investigation of the regenerative mechanism of liver regeneration after partial hepatectomy in mice
    LU Mei-xin, ZHANG Ming-ming, ZHAO Ling-ping, ZENG Quan, XI Jia-fei, PEI Xue-tao, WANG Hai-yang, YUE Wen
    2022, 46(9): 659-667. https://doi.org/10.7644/j.issn.1674-9960.2022.09.004
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Objective To investigate the initiation mechanism of liver regeneration by establishing a mouse model of liver regeneration after partial hepatectomy （PH）. Methods PH surgeries were performed on mice. The tissue samples were collected at 12, 24, 36, 48 and 72 h post operation,and a sham-operated group was set up as a control. The pathological changes, apoptotic signals, BrdU incorporation, and expressions of proliferation-related genes were detected in the regenerating liver tissues. To find out about the time point of initiation of regeneration, bioinformatic analysis of the single-cell transcriptome data from the above liver tissues was performed and the cell cycle distribution of epithelial cells was assessed. Moreover, a secretion profile of hepatic stellate cells （HSCs） during regeneration was analyzed to investigate the regeneration-related secreted factors. A co-culture assay was performed in vitro, and the effect of HSCs on hepatocytes proliferation was detected by quantitative real-time PCR and immunofluorescence staining. Results A mouse PH model was constructed. The BrdU incorporation levels and the quantitative real-time PCR results showed that the mouse liver rarely proliferated at the early stage （within 24 h） after PH, but peaked at 36-48 h after PH. Transcriptome analysis at the single cell level showed that a large number of epithelial cells entered the S phase at 48 h after PH; The secretion profile obtained from HSCs at the early stage of regeneration revealed a spectrum of secretory factors including Fgf9, which was possibly involved in the initiation of liver regeneration. In vitro co-culture assays showed that LX-2 cells could promote the proliferation of THLE-2 cells. Conclusion During the early stage of liver regeneration after PH, HSCs may possibly secrete a variety of factors with pro-proliferation functions. Also, the HSCs cultured in vitro are able to promote the proliferation of hepatocytes. HSCs might participate in the initiation of liver regeneration and play a positive regulatory role.
  • Original articles
    USP13 knockdown promotes apoptosis of hepatocytes induced by TNF-α
    LIU Kai, WANG Ting, CHEN Xu, LI Ke-ying, XIANG Shen-si, REN Guang-ming, YANG Xiao-ming
    2022, 46(9): 668-672. https://doi.org/10.7644/j.issn.1674-9960.2022.06.005
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Objective To investigate the effect of ubiquitin specific proteases 13（USP13） knockdown on apoptosis of hepatocytes induced by TNF-α. Methods Primary hepatocytes of mice were isolated and cultured in vitro and treated with TNF-α or TNF-α and cycloheximide （CHX）. MTS assay was used to detect cell viability while flow cytometry was performed to measure cell apoptosis. The mRNA level of apoptosis-related genes was detected by qRT-PCR. Caspase-3 apoptosis kit was used to detect the levels of caspase-3 activation. Western blotting was used to detect the activation of NF-κB and MAPK signaling pathways. Results Treatment with TNF-α and CHX decreased cell viability but increased apoptosis in USP13 knockout mouse hepatocytes. Mechanism study showed that knockout of USP13 resulted in up-regulation of pro-apoptotic genes and down-regulation of anti-apoptotic genes, enhancing the activation of caspase-3 under the co-treatment with TNF-α and CHX. Moreover, knockdown of USP13 prevented TNF-α-induced activation of NF-κB and MAPK signaling pathways. Conclusion Deficiency of USP13 in mouse hepatocytes inhibits the activation of NF-κB and MAPK signaling pathways and increases hepatocyte apoptosis induced by TNF-α.
  • Original articles
    Preparation and identification of polyclonal antibodies against mouse interferon regulatory factor 7
    YANG Xi-qin, ZOU Tao, ZHANG Yao-lin, ZHANG Ji-yan
    2022, 46(9): 673-676. https://doi.org/10.7644/j.issn.1674-9960.2022.09.006
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Objective To obtain the protein of recombinant interferon regulatory factor 7 （IRF7） of mice, and prepare and identify anti-IRF7 polyclonal antibodies. Methods The pET-28a（+）-IRF7 plasmid was constructed, IRF7 was expressed in E.coli and purified with Ni sepharose. Rabbits were immunized by the purified protein with MnJ and Freund's adjuvant respectively. The antisera were collected and the titers of anti-IRF7 antibodies were determined by indirect ELISA.The antibodies were purified by Melon™ Gel Monoclonal IgG Purification Kit. The biological activities of the antibodies were assessed by Western blotting and immunoprecipitation. Results The IRF7 protein was purified by Ni sepharose. The titers of polyclonal antibodies prepared by the two adjuvants were 1∶; 810×; 103. The polyclonal antibodies were obtained by the antibody purification kit. The eukaryotically expressed IRF7 could be specifically identified by the two polyclonal antibodies,and these antibodies could be used in immunoprecipitation assay. The two polyclonal antibodies were significantly better at precipitation effect than commercial ones. Conclusion The polyclonal antibodies against IRF7 are successfully prepared, which can contribute to studies on the functions of IRF7 and related mechanisms.
  • Original articles
    Construction and motility analysis of residue sites of bacterial outer membrane protein BamA mutants
    WANG Yuan, LI Zhi-hui, WEI Qian, DONG Guo-fu, Chu Yin-di, WANG Chang-zhen
    2022, 46(9): 677-681. https://doi.org/10.7644/j.issn.1674-9960.2022.09.007
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Objective To construct a prokaryotic expression vector of β-barrel assembly machinery A （BamA） mutants, and analyze the mobility of residue site glycine （G） 313 in the polypeptide transport-associated domain （POTRA） of BamA. Methods Site-directed mutagenesis was performed using sequence overlapped extension PCR （SOE-PCR）. Two natural cysteines at sites 690 and 700 were mutated to serine （S） while the glycine （G） at site 313 in the POTRA domain was mutated to cycteine （C） so that the prokaryotic expression vector pET22b-Strep-BamA containing C690S-C700S-G313C was constructed. The BamA mutants were induced with isopropyl β-D-thiogagactopyranosid （IPTG） and the expression product was purfied using Strep-Tactin Sepharose. The cysteine was labeled with 2,2,5,5-tetramethyl-1-oxyl-3-methyl methanethiosulfonate（MTSL） for electron paramagnetic resonance （EPR） experiments. The rotational correlation time τc of the EPR spectrum was calculated in order to analyze the mobility characteristics of G313C amino acid. Results The prokaryotic expression vector pET22b-Strep-BamA containing mutated sites was constructed. The mutant protein was efficiently expressed and purified. The EPR spectrum of G313C site presented as a single-component spectrum. The τc value of G313C site was calculated as （2.30± 0.03） ns. Conclusion The BamA recombinant mutant protein is expressed and purified in a prokaryotic expression system. The results reveal the mobility characteristics of G313C amino acid site and provide data for future studies on the structural mechanism of BamA in the process of outer membrane protein （OMP） integration.
  • Original articles
    Screening and verification of affinity peptide for SARS-CoV-2 spike 1 protein
    YANG Fang-fang, LONG Jin-rong, WANG Xin, LI Lei, CAO Yi-ming, YANG Jing, WANG Sheng-qi
    2022, 46(9): 682-685. https://doi.org/10.7644/j.issn.1674-9960.2022.09.008
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Objective To use SARS-CoV-2 spike protein （S protein） as the target to screen anti-SARS-CoV-2-peptide drugs. Methods The SARS-CoV-2 spike protein binding peptides were screened out through affinity selection from a 12-peptide display library, the affinity between the peptides and S protein was verified by enzyme linked immunosorbent assay （ELISA）, and the anti-SARS-CoV-2 activity was evaluated in HEK-293T-ACE2 cells. Results p27 had a strong affinity with S1 protein and could prevent SARS-CoV-2 pseudovirus from entering cells with IC50 of 73 μmol/L. Conclusion Peptide p27 may have anti-SARS-COV-2 activity and can be potentially developed into anti-SARS-CoV-2.
  • Original articles
    Hyperbaric oxygen alleviates renal ischemia-reperfusion injury in rats
    LI Jun, WANG Ning, DOU Jian-Ping, LIU Li-li
    2022, 46(9): 686-690. https://doi.org/10.7644/j.issn.1674-9960.2022.09.009
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Objective To observe the therapeutic effect of hyperbaric oxygen （HBO） therapy against the renal ischemia-reperfusion injury （I/R）model in rats and the underlying mechanism. Methods Twenty-four adult male SD rats were randomly divided into three groups（n=8 each）：sham operated group（Sham group）,ischemia-reperfusion injury group（I/R group）and ischemia-reperfusion injury combined with HBO （I/R+HBO group）. Bilateral renal arteries were ligated for 60 min in the I/R group, 2.5 ATAT HBO 60 min method was used in the I/R+HBO group, and only incision and suture were given in the Sham group. All rats were harvested at 12 h after operation. The changes in renal function, renal structure, apoptosis and autophagy pathway activation were observed in each group. Results Compared with the Sham group, the levels of urea nitrogen （BUN） and creatinine （Cr）in the I/R group increased significantly at 12 h after operation. PAS staining showed that the renal tubular structure was seriously damaged with a large number of epithelial cells shed and some casts. TUNEL showed that brown staining particles increased significantly at the same time. Furthermore, compared with the I/R group, the levels of BUN and Cr in the I/R+HBO group decreased significantly at 12 h after operation. The renal tubular injury, casts and brown stained cells were decreased simultaneously. The mRNA expressions of HIF-1α and BNIP3 were both up-regulated in the I/R+HBO group. Immunohistochemical staining showed that the expression distribution of LC3 in the renal tubular region was up-regulated. Conclusion HBO can alleviate renal I/R injury and renal function decline,reduce renal tubular cell injury and cell apoptosis in rats, which may be related to the autophagy pathway activation in the kidney after HBO treatment.
  • Original articles
    Research on the mechanism of anti-influenza effect of Moslae Herba based on network pharmacology
    JIN Ya-qing, ZU Mian, HONG Yong-kai, LI Li-juan
    2022, 46(9): 691-698. https://doi.org/10.7644/j.issn.1674-9960.2022.09.010
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Objective To analyze the potential active ingredients, targets and mechanism of anti-influenza effect of Moslae Herba using network pharmacology methods. Methods The chemical composition of Moslae Herba was retrieved from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform（TCMSP）. The potential targets of compounds were collected from the SwissTargetPrediction database, and the influenza targets were retrieved by GeneCards, OMIM database and literature mining. The ingredients-targets′ network was constructed with Cytoscape, and the protein-protein interactions （PPIs） of intersection targets were obtained from the STRING database and imported into Cytoscape. The intersection targets′ enrichment analysis of gene ontology （GO） and Kyoto Encyclopedia of Genes and Genomes （KEGG） was carried out by Metascape. Results Fourteen active ingredients and 243 potential targets of Moslae Herba were found and 1088 influenza-related targets were obtained. There were 45 targets where the components of the Moslae Herba and influenza intersected. There were 14 effective active compounds of Moslae Herba that were effective influenza, among which the six key ingredients were chryseriol, luteolin, kaempferol, kumatakenin, acacetin and quercetin. PPI network analysis resulted in nine critical target proteins,including Akt1, SRC, HSP90AA1 and EGFR. Metascape gene enrichment analysis resulted in 708 GO terms, which were related to multiple biological processes such as cytokines, immunomodulation and oxidative stress. One hundred and thirty KEGG pathways were also obtained through gene enrichment analysis, involving pathogen infection, PI3K-Akt signaling pathway, chemokine signaling pathway and phospholipase D signaling pathway. Conclusion This study reveals that Moslae Herba may help treat influenza via its anti-inflammatory, antiviral and immunomodulatory proporties, which provides valuable reference for future experimental studies.
  • Original articles
    VFA： a factor analysis algorithm for single-cell transcriptomic data based on the deep generative model
    LIU Run-yan, AN Si-jing, HU Shuo-feng, CHEN Yao-wen, DONG Guo-hua, MEN Ya-hui, HE Zhen, YING Xiao-min
    2022, 46(9): 699-704. https://doi.org/10.7644/j.issn.1674-9960.2022.09.011
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Objective To propose a deep generative model based factor analysis method for single-cell transcriptomic data, and explain the model results by exploring biological heterogeneity from both cell-and gene-levels. Methods A deep generative model for factor analysis of single-cell transcriptomics data was established, which was trained using the transcriptomic data of mouse embryonic developmental cells. Downstream biological analysis on the results was performed, such as annotation of cell types and visualization of factors and loadings. Results The model achieved dimensionality reduction of single-cell transcriptomic data while retaining the heterogeneity. The factors obtained from model training recognized features of different cell types, and the factor loadings identified cell-type-specific genes. Conclusion This factor analysis method based on the deep generative model has better interpretability, which can extract representative information from complex and intricate single-cell transcriptomic data,and interpret biological meanings at different levels.
    • Reviews
  • Reviews
    The influence of microgravity on brain cognition and improvement strategies
    LIU Zi-wei, LI Qi, YUAN Bo-chuan, DU Li-na, JIN Yi-guang
    2022, 46(9): 705-710. https://doi.org/10.7644/j.issn.1674-9960.2022.09.012
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    With the rapid development of aerospace in China, the influence of the space environment on astronauts has attracted more attention. Microgravity is extremely important for cognitive ability of the brain, which is closely related to the successful completion of the spaceflight mission. Therefore, the negative influence of microgravity on cognitive ability is an important issue to be solved urgently in space medicine. This review summarizes the effects of microgravity on cognition, possible mechanisms, and ways to reduce the negative effects of microgravity. It is hoped that this study will provide support for the aerospace development of China.
  • Reviews
    Research progress in mechanisms of Clostridium perfringens α toxin
    GUO Zi-shuo, XIN Wen-wen, WANG Jing-lin
    2022, 46(9): 711-714. https://doi.org/10.7644/j.issn.1674-9960.2022.09.013
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Alpha toxin, one of the main virulence factors of Clostridium perfringens, is capable of two enzymatic activities, phospholipase C and sphingomyelinase. Alpha toxin can hydrolyze phosphatidylcholine and sphingomyelin on cell membranes, resulting in a variety of human and animal diseases. At the same time, α toxin is also a potential biological warfare agent, which is of great significance for national biosecurity. This paper provides an overview of the biological structure and properties of C.perfringens α toxin, signaling pathways of toxin cell toxicity,molecular mechanisms of platelet activation, and therapeutic value.
  • Reviews
    Research progress in the effect of oral and nasal microorganisms on neurodegenerative diseases
    LIU Shui-rong, XU Zhen-hua, JIANG Xiao-xia
    2022, 46(9): 715-719. https://doi.org/10.7644/j.issn.1674-9960.2022.09.014
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    From a plague to neocoronavirus, microorganisms have impacted human health. At the same time, human beings have never stopped studying microorganisms.Studies have shown that microorganisms living in the gut and other parts can affect the nervous system by migrating or secreting secondary metabolites. Therefore, researchers proposed the concept of the gut brain axis. In recent years, it has been found that in addition to intestinal microorganisms, those living in the mouth and nose also have an impact on the nervous system.Alzheimer′s disease（AD） and Parkinson′s disease（PD） are the two most common neurodegenerative diseases.This paper reviews the effects of oral and nasal microorganisms on AD and PD, and offers some novel ideas about the treatment of related diseases in the future.
Journal Online
News
More>>
Download
More>>
Links
More>>
Visited
  • Total visitors:
    Visitors of today:
    Now online: