Military Medicine
ZHANG Zi-nan, MA Jia-jia, HUANG Jing-yi, XING Huan-chun, LI Yao, WANG Yong-an, DING Ri-gao, YANG Jun
Objective To assess the toxic effects of different doses of nerve agent tabun on the cholinergic system in mice and to explore the related patterns. Methods Mice were subcutaneously injected with different doses of tabun （100, 200,350, and 500 μg/kg）. Body weight and brain weight were recorded at different time points（10 min,1 h,4 h,24 h, 48 h, and 72 h）, and organ coefficients were calculated. The activity of AChE was determined using the Ellman method.ELISA was used to determine the content of AChE. Results The body weight of severely exposed mice was significantly lower than that of other groups after 24 h. The inhibitory rates of different concentrations of tabun on blood enzymes decreased significantly within 48 h, but increased to some extent at 72 h. The inhibitory rate increased significantly at 24 h after mild exposure. Under moderate and severe exposure, the inhibitory rate of blood enzymes remained at a high level for 1 h and then decreased significantly. The inhibitory state of brain enzymes was significantly different from that of blood enzymes. Under severe exposure, the high inhibitory rate of brain enzymes increased within 4 h and decreased significantly after a high inhibitory state was maintained within 24 h. The inhibitory rate of brain enzymes were similar under moderate and mild exposure, but did not vary significantly with the change in exposure doses. After 48 h, the inhibitory rates of brain enzymes in both exposure states trended up, but were still significantly lower than in the severe exposure group. Both brain enzymes and blood enzymes trended up for some time after administration. Conclusion Under mild or moderate poisoning, the toxic agent has little inhibitory effect on central AChE, but has inhibitory effect at a high dose. Therefore, the inhibitory rate of blood AChE is of referential value and can help measure the severity of poisoning. At the same time, experimental results suggest that we need to pay attention to the large amount of poison retained in the peripheral area after exposure. During the treatment of poisoning, multiple administration should be used to minimize the effect of poison.
